Mechanisms of Action of Islet Neogenesis Associated Protein 1 (ingap): Comparison of the Full-length Recombinant Protein

MECHANISMS OF ACTION OF ISLET NEOGENESIS ASSOCIATED PROTEIN 1 (INGAP): COMPARISON OF THE FULL-LENGTH RECOMBINANT PROTEIN AND 2 A BIO-ACTIVE PEPTIDE 3 4 Maria Petropavlovskaia, Jamal Daoud, Jonathan Zhu, Mandana Moosavi, Jieping Ding, 5 Julia Makhlin, Beatrice Assouline-Thomas, and Lawrence Rosenberg 6 Department of Surgery, McGill University, the Research Institute of the McGill University 7 Health Center, and The Lady Davis Research Institute of the Jewish General Hospital 8 McGill University, Montreal, Quebec, Canada 9 10 Author contributions: 11 M.P. designed the study, performed most experiments and wrote the manuscript; J.D. performed 12 confocal imaging; J.ZImmunoblotting and BrdU staining; M.M.-BrdU staining experiments; 13 J.D., J.M., B.A-T. prepared rINGAP and carried out a few experiments. LR.oversaw the 14 project. 15 16 Abbreviated title: Mechanisms of action of INGAPprotein and INGAP-peptide 17 18 Address correspondence and reprint request to: Maria Petropavlovskaia, JGH/LDI, 3755 19 Cote Ste-Catherine Rd, Rm F-130 Montreal, Quebec, Canada, H3T 1E2. 20 e-mail: [email protected] 21 22 Disclosure summary: Authors have no conflicts of interest 23 Articles in PresS. Am J Physiol Endocrinol Metab (July 31, 2012). doi:10.1152/ajpendo.00670.2011